P8240
REDExtract-N-Amp™ PCR ReadyMix™ for Blood
12 mL sufficient for 1000 amplifications
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.55
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Quality Level
usage
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
feature
dNTPs included
hotstart
color
red
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
REDExtract-N-Amp™ PCR ReadyMix™ for Blood is a PCR master mix containing buffer, salts, dNTPs, and Taq polymerase. This 2x reaction mixture also contains JumpStart™ Taq antibody for specific hot start amplification and REDTaq® dye, an inert tracking dye to enable direct loading of PCR products onto an agarose gel for analysis. PCR ReadyMix™ is intended for use with Sigma′s Extract-N-Amp™ Blood PCR Kits. All Extract-N-Amp™ Kit include a volume of PCR ReadyMix sufficient for at least one PCR reaction per extraction. However, where additional amplifications are required, supplemental PCR ReadyMix may be needed.
Application
REDExtract-N-Amp™ PCR ReadyMix™ for Blood has been used for polymerase chain reaction (PCR).
Features and Benefits
- Additional loading dye is not required as the PCR reaction mix contains REDTaq® dye for direct and convenient loading onto an agarose gel for analysis
- Hotstart feature allows for room temperature set-up without premature Taq DNA polymerase activity
- Ready-mix enables time-saving procedures
- Reduced risk of contamination from multiple pipet steps and increased reproducibility
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sofia Lejon Crottet et al.
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 50(2), 169-174 (2014-04-01)
Starting in 2013, blood donors must be tested at least using: (1) one monoclonal anti-D and one anti-CDE (alternatively full RhCcEe phenotyping), and (2) all RhD negative donors must be tested for RHD exons 5 and 10 plus one further
Protocols
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
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