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P3169

Sigma-Aldrich

pGlu-Phe-Leu p-nitroanilide

protease substrate, ≥98% (TLC), powder

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About This Item

Empirical Formula (Hill Notation):
C26H31N5O6
CAS Number:
85901-57-1
Molecular Weight:
509.55
MDL number:
MFCD00080242
UNSPSC Code:
12352204
PubChem Substance ID:
24898399
NACRES:
NA.32

product name

pGlu-Phe-Leu p-nitroanilide, protease substrate

Quality Level

100

Assay

≥98% (TLC)

form

powder

solubility

ethanol: 10 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CC(C)CC(NC(=O)C(Cc1ccccc1)NC(=O)C2CCC(=O)N2)C(=O)Nc3ccc(cc3)N(=O)=O

InChI

1S/C26H31N5O6/c1-16(2)14-21(25(34)27-18-8-10-19(11-9-18)31(36)37)29-26(35)22(15-17-6-4-3-5-7-17)30-24(33)20-12-13-23(32)28-20/h3-11,16,20-22H,12-15H2,1-2H3,(H,27,34)(H,28,32)(H,29,35)(H,30,33)

InChI key

NLVMZXVGZDVXDA-UHFFFAOYSA-N

Substrates

Chromogenic substrate for thiol proteases such as papain, ficin and bromelain.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Filippova IYu et al.
Analytical biochemistry, 143(2), 293-297 (1984-12-01)
L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA)--a convenient chromogenic substrate for assay of thiol proteinases papain, ficin, and bromelain--was prepared by enzymatic synthesis with chymotrypsin as a catalyst. The thiol proteinases hydrolyze PFLNA with the liberation of p-nitroaniline, estimated spectrophotometrically by its absorbance at 410
Rim Hamza et al.
BMC plant biology, 18(1), 24-24 (2018-01-27)
Plants and insects have coexisted for million years and evolved a set of interactions which affect both organisms at different levels. Plants have developed various morphological and biochemical adaptations to cope with herbivores attacks. However, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)
Dalgys Martínez et al.
International journal of molecular sciences, 20(12) (2019-06-27)
The house dust mite (HDM) Dermatophagoides pteronyssinus is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that is based on recombinant proteins has been proposed for the safer and more efficient treatment of allergic diseases. The aim
Rim Hamza et al.
Bio-protocol, 8(17), e2993-e2993 (2018-09-05)
Enzymes play a key role in insect-plant relationships. For a better understanding of these interactions, we analyzed Tuta absoluta digestive enzymes. Here, we describe a detailed protocol for the detection of trypsin and papain-like enzymes in Tuta absoluta larvae by

Articles

Papain

Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

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