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P2459

Sigma-Aldrich

Phenyl-Sepharose 6 Fast Flow

high substitution, extent of labeling: ~40 μmol per mL (high substitution)

Synonym(s):

Phenyl-Agarose

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About This Item

MDL number:
UNSPSC Code:
41106500
NACRES:
NA.56

Quality Level

form

suspension

extent of labeling

~40 μmol per mL (high substitution)

matrix

agarose, 6% cross-linked

matrix activation

epichlorohydrin

matrix attachment

ether

matrix spacer

3 atoms

particle size

45-165 μm

storage temp.

2-8°C

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General description

P2459-200ML′s updated product is GE17-0973-05

Application

Phenyl Sepharose is used in protein chromatography, affinity chromatography, hydrophobic interaction media, resins and separation media. Phenyl Sepharose has been used in studies that contributed to improving industrial applications in additives in detergents and feed industries. Phenyl Sepharose has also been used to study microbial communities inhabiting hypersaline environments.

Physical form

Suspension in 20% ethanol
aqueous ethanol suspension

Legal Information

Sepharose is a trademark of Cytiva

related product

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

104.0 °F

Flash Point(C)

40 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Frank J Ruzicka et al.
Biochimica et biophysica acta, 1774(2), 286-296 (2007-01-16)
A gene eam in Clostridium difficile encodes a protein that is homologous to lysine 2,3-aminomutase (LAM) in many other species but does not have the lysyl-binding residues Asp293 and Asp330 in LAM from Clostridium subterminale SB4. The C. difficile protein
<I>Candidaguilliermondii</I> grows on rare pentoses ? implications for production of pure xylitol.
Granstrom, T., et al.
Biotechnology Letters, 24, 507-510 (2002)
Dawei Chen et al.
Biochemistry, 45(42), 12647-12653 (2006-10-18)
Lysine 2,3-aminomutase (LAM) from Clostridium subterminale SB4 catalyzes the interconversion of (S)-lysine and (S)-beta-lysine by a radical mechanism involving coenzymatic actions of S-adenosylmethionine (SAM), a [4Fe-4S] cluster, and pyridoxal 5'-phosphate (PLP). The enzyme contains a number of conserved acidic residues
K A Rye et al.
The Journal of biological chemistry, 270(1), 189-196 (1995-01-06)
The effect of cholesteryl ester transfer protein (CETP) on the size, composition, and structure of spherical, reconstituted HDL (rHDL) which contain apolipoprotein (apo) A-I as their sole apolipoprotein has been studied. Spherical rHDL were incubated with CETP and Intralipid for
K A Rye et al.
The Journal of biological chemistry, 271(8), 4243-4250 (1996-02-23)
The effect of sphingomyelin (SPM) on the structure and function of discoidal and spherical reconstituted high density lipoproteins (rHDL) has been studied. Three preparations of discoidal rHDL with 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC)/SPM/unesterified cholesterol (UC)/apolipoprotein (apo)A-I molar ratios of 99.6/0. 0/10.2/1.0, 86.0/13.6/10.8/1.0

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