Skip to Content
Merck
All Photos(1)

Documents

P1473

Sigma-Aldrich

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

Synonym(s):

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41141827
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Related Categories

General description

Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Application

PCR 100 bp Low Ladder has been used:
  • in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis
  • to visualize DNA from enzyme digested PCR mixture
  • to estimate the molecular weight of the PCR product fragments

Components

Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
For optimal resolution, the recommended agarose gel concentration is 2.0%.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 4

1 of 4

DNA Molecular Weight Marker XIII (50 bp ladder) pkg of 50 μg (250 μg/ml), solution

Roche

11721925001

DNA Molecular Weight Marker XIII (50 bp ladder)

Gel Loading Buffer for NA electrophoresis

Sigma-Aldrich

G2526

Gel Loading Buffer

Gel Loading Solution for NA electrophoresis, solution

Sigma-Aldrich

G7654

Gel Loading Solution

SYBR® Green I nucleic acid gel stain 10,000 × in DMSO

Sigma-Aldrich

S9430

SYBR® Green I nucleic acid gel stain

The relationships between performance traits and the bGH/Alu I and Pit-1/Hinf Ipolymorphisms in Holstein cows
Ozdemir M, et al.
Indian Journal of Animal Sciences (India) , 52, 186-191 null
Gbenga A Adewumi et al.
Frontiers in microbiology, 3, 436-436 (2013-01-15)
In this study, bacterial composition of iru produced by natural, uncontrolled fermentation of Parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques. PCR-denaturing gradient gel electrophoresis (DGGE) revealed similarity in DNA fragments with the
Susan Walker et al.
PloS one, 9(9), e106262-e106262 (2014-09-04)
In human adults, and animals, the Apelin-APJ ligand-receptor system is emerging as having a role in the pathogenesis of cardiovascular function and heart failure. The aim was to investigate expression, and regulation by oxygen, of the Apelin APJ receptor (APJ)

Articles

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service