N4888
Nutrient Mixture F-12 Ham
With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Ham’s F-12
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About This Item
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
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General description
Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.
Application
Nutrient Mixture F-12 Ham has been used:
- in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
- to culture female-originated neuroblastoma cells
- to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines
Reconstitution
Supplement with 0.146 g/L L-glutamine.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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