MAK466
Free Fatty Acid Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12161503
Recommended Products
Quality Level
application(s)
agriculture
food and beverages
pharmaceutical
detection method
colorimetric
fluorometric
relevant disease(s)
diabetes; pediatric diseases; endocrinological disorders; neurological disorders
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Fatty acids are aliphatic monocarboxylic acids ubiquitously found in animal or vegetable fat, oil, and wax. Fatty acids play important roles in cellular synthesis, energy metabolism, and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome, and Reye Syndrome.
Features and Benefits
- Linear detection range: colorimetric assay 7 - 1000 µM, fluorometric assay 7 - 100 µM fatty acid.
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for detecting free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, and cell cultures, and in food and agriculture products, as well as studying the effects of drugs on free fatty acid metabolism.
Principle
The Free Fatty Acid Assay Kit provides a simple, one-step and high-throughput assay for measuring Free Fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity (λEx = 530 nm/λEm = 585 nm) is directly proportional to the free fatty acid concentration in the sample.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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