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M7802

Sigma-Aldrich

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ERK, Anti-ERK-2, Anti-ERK2, Anti-ERT1, Anti-MAPK2, Anti-NS13, Anti-P42MAPK, Anti-PRKM1, Anti-PRKM2, Anti-p38, Anti-p40, Anti-p41, Anti-p41mapk, Anti-p42-MAPK

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About This Item

MDL number:
MFCD01633612
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ERK-PT115, monoclonal

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

species reactivity

rat, human

concentration

~2 mg/mL

technique(s)

capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol

isotype

IgG1

UniProt accession no.

P28482

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPK1(5594) , MAPK3(5595)
rat ... Mapk1(116590) , Mapk3(50689)

General description

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Specificity

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr183 in ERK-2).

Immunogen

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Application

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
  • immunoblotting
  • western blotting
  • immunofluorescence
  • enzyme linked immunosorbent assay (ELISA)
  • immunochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of the role of MAPK1 and CREB1 polymorphisms on treatment resistance, response and remission in mood disorder patients
Calati R, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 44, 271-278 (2013)
Leader cell positioning drives wound-directed collective migration in TGFbeta-stimulated epithelial sheets
Chapnick DA and Liu X
Molecular Biology of the Cell, 25(10), 1586-1593 (2014)
Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis
Huang YWA, et al.
Cell, 148(5), 933-946 (2012)
Transcriptional consequences of 16p11. 2 deletion and duplication in mouse cortex and multiplex autism families
Blumenthal I, et al.
American Journal of Human Genetics, 94(6), 870-883 (2014)
MAPKs: new JNK expands the group
Davis RJ
Trends in Biochemical Sciences, 19, 1027-1037 (1994)
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