L4277
Lipase from Aspergillus oryzae
≥20,000 U/g
Synonym(s):
Palatase® 20,000L
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About This Item
Recommended Products
form
lyophilized
Quality Level
specific activity
≥20,000 U/g
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
General description
Rhizomucor miehei lipase is a carboxylesterase and belongs to the α/β-hydrolase family. It comprises a lid domain, hinge domain and a catalytic triad Ser?His?Asp/Glu. The three-dimensional structure is a α/β hydrolase fold which is very similar as that of esterase enzyme.
Application
Lipase from Rhizomuco miehei has been used:
- in the partial digestion of triacylglycerols (TAG)
- in the digestion of (12-ricinoleoylricinoleoyl)diricinoleoylglycerol (RRRR) present in castor oil
- in the hydrolysis of linseed oil
Biochem/physiol Actions
Lipase catalyzes the hydrolysis of monoacylglycerols and diacylglycerols and its activity is inhibited by divalent actions. Rhizomuco miehei lipase is an industrial enzyme with application in food, detergent and pharmaceutical industries. It is also used in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis. Rhizomuco miehei lipase may have potential in producing biodiesel from waste cooking oil.
Preparation Note
purified 1,3-specific lipase from Rhizomucor miehei produced by submerged fermentation of a genetically modified Rhizomucor miehei microorganism
Legal Information
A product of Novozyme corp.
Palatase is a registered trademark of Novozymes Corp.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Jiann-Tsyh Lin et al.
Journal of agricultural and food chemistry, 56(10), 3616-3622 (2008-04-30)
(12-Ricinoleoylricinoleoyl)diricinoleoylglycerol (RRRR), a tetraacylglycerol, was identified earlier in castor oil. Using ESI-MS (4), 95% of the 12-ricinoleoylricinoleoyl chain was identified at the sn-2 position of the glycerol backbone of RRRR. Regiospecific location of the 12-ricinoleoylricinoleoyl chain of RRRR on the
Xianming Zhao et al.
Frontiers in microbiology, 10, 645-645 (2019-04-12)
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Thais de Andrade Silva et al.
Scientific reports, 12(1), 6815-6815 (2022-04-28)
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Jurnal Ilmu Dasar, 9(1), 8-Jan-8-Jan (2008)
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