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H9658

Sigma-Aldrich

Monoclonal Anti-HA antibody produced in mouse

clone HA-7, ascites fluid

Synonym(s):

Monoclonal Anti-HA, HA Antibody - Monoclonal Anti-HA antibody produced in mouse, Ha Antibody Sigma, Sigma Ha Antibody, Anti-HA, Anti-Influenza Hemagglutinin

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About This Item

MDL number:
MFCD00803873
UNSPSC Code:
12352203

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HA-7, monoclonal

contains

15 mM sodium azide

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:
  • immunoprecipitation in human embryonic kidney 293 cells
  • immunoblotting in HeLa cells
  • immunopurification in human embryonic kidney 293 cells
  • immunostaining for electron microscopy in mouse embryonic fibroblasts
  • in immunocytochemistry of hippocampal slices
Monoclonal Anti-HA antibody produced in mouse has been used in:
  • Immunoblotting, to study the expression of fusion protein
  • Coimmunoprecipitation assay
  • Immunoblotting of lysate protein

Biochem/physiol Actions

Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.
Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Receptor binding and pH stability?how influenza A virus hemagglutinin affects host-specific virus infection
Mair CM, et al.
Biochimica et Biophysica Acta - Biomembranes, 1838(4), 1153-1168 (2014)
Analysis of influenza virus hemagglutinin receptor binding mutants with limited receptor recognition properties and conditional replication characteristics
Bradley KC, et al.
Journal of Virology, 85(23), 12387-12398 (2011)
The role of influenza A virus hemagglutinin residues 226 and 228 in receptor specificity and host range restriction.
Vines A, et al.
Journal of Virology, 72(9), 7626-7631 (1998)
Synaptic targeting and functional modulation of GluK1 kainate receptors by the auxiliary neuropilin and tolloid-like (NETO) proteins.
Copits B A, et al.
The Journal of Neuroscience, 31(20), 7334-7340 (2011)
Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency Situations.
Metwally A and Yousif A
International Journal of Molecular Sciences, 18(4), 766-766 (2017)
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