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form
solid
feature
wetted part: no
packaging
pack of 25 g
manufacturer/tradename
Cytiva RPN6302
color
Yellowish
solubility
cold water: soluble (Easily soluble in cold and hot water)
storage temp.
2-8°C
Related Categories
General description
Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.
Application
Iodoacetamide has been used:
- as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
- in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
- as a protein alkylation agent
Biochem/physiol Actions
Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Features and Benefits
- Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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