GE29-0486-09
GSTrap™ 4B
Cytiva 29-0486-09, pack of 1 mL
About This Item
Recommended Products
ligand
glutathione
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0486-09
availability
not available in North America
parameter
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi (H2O at 25°C)
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)(H2O at 25°C)
Related Categories
General description
Application
Features and Benefits
- High binding capacity.
- Mild elution conditions preserving protein antigenicity and function.
- Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
- GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as AKTA design.
Storage and Stability
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Protocols
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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