GE17-5110-01
HiTrap® IGM purification
Cytiva 17-5110-01, pack of 5 × 1 mL
About This Item
Recommended Products
ligand
2-mercaptopyridine
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5110-01
storage condition
(20% Ethanol)
parameter
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-13
working range
3-11
storage temp.
2-8°C
Related Categories
General description
Application
Features and Benefits
- Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
- Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
- Recovery of IgM is high with excellent activity retention.
- The top and bottom frits are manufactured from porous polyethylene
- Columns are delivered with a stopper on the inlet and a snap-off end on the outle
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to prepare samples for purification with affinity chromatography.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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