GE17-0729-10
SP Sepharose™ Fast Flow
Cytiva 17-0729-10, pack of 25 mL
About This Item
Recommended Products
ligand
sulphopropyl
description
Ion Exchanger Type (value)
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0729-10
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
3-14
working range
4-13
capacity
70 mg binding capacity(ribonuclease A/ml medium)
suitability
suitable for bioprocess medium
Related Categories
General description
Application
As member of the BioProcess media range, SP Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Well-proven strong cation exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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