GE17-0409-03
HiTrap® Chelating High Performance
Cytiva 17-0409-03, pack of 5 × 5 mL
About This Item
Recommended Products
ligand
iminodiacetic acid
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0409-03
parameter
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
size
avg. part. size
34 μm
cleaning
2-14
working range
3-13
Related Categories
General description
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC™ Systems.
Features and Benefits
- For optimizing purification of histidine-tagged proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap™ Kit, HiTrap® Chelating HP and Chelating Sepharose® Fast Flow products from Cytiva.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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