WI 38 Cell Line human
90020107, caucasian fibroblast-like fetal lung cell
Synonym(s):
WI38 Cells, Wistar Institute-38 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human lung (fetal)
growth mode
Adherent
karyotype
2n = 46, diploid except at high passage number
morphology
Fibroblast-like
products
Collagen
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian foetal lung
Cell Line Description
From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.
Application
From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.
Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.
Virus studies (rhinoviruses), vaccine production
WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.
Biochem/physiol Actions
Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.
Analysis Note
Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS
Other Notes
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