RPMI 8226
87012702
Synonym(s):
RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human blood
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Immunoglobulin G (Ig) lambda light chain
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human myeloma
Cell Line Description
Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.
Application
RPMI 8226 has been used:
- to grow human myeloma cell lines
- to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
- to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.
Other Notes
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Certificates of Analysis (COA)
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