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recombinant
expressed in E. coli
Quality Level
form
liquid
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
selection
blasticidin
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
This product is a neomycin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link above to order gRNA.
Application
Functional Genomics/Screening /Target Validation/Genome Editing
Features and Benefits
Use lentiviral particles to generate cells with stable expression of Cas9 nuclease.
Components
1ug of plasmid DNA, EF1a-Cas9-2A-Neomycin Lenti
Linkage
Click here to order gRNA plasmid and virus.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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