C-12642
Human Small Airway Epithelial Cells (HSAEpC)
500,000 cryopreserved cells
Synonym(s):
HSAEpC cells
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About This Item
UNSPSC Code:
41106514
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biological source
human (bronchiole)
packaging
pkg of 500,000 cells
morphology
(epithelial)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
asthma
shipped in
dry ice
storage temp.
−196°C
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General description
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Human Small Airway Epithelial Cells (HSAEpC) serve as model cell culture systems for in vitro immune response studies. The 3D culture of HSAEpC mimics the in vivo cells effectively. The 3D cells are more viable, functional and efficient for immune response studies compared to the 2D cells.
Human Small Airway Epithelial Cells (HSAEpC) serve as model cell culture systems for in vitro immune response studies. The 3D culture of HSAEpC mimics the in vivo cells effectively. The 3D cells are more viable, functional and efficient for immune response studies compared to the 2D cells.
Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area and are provided in a cryopreserved format. They stain positive for cytokeratin.The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.
Cell Line Origin
Bronchiole
Application
Human Small Airway Epithelial Cells (HSAEpC) may be used:
- in overexpression of αvβ6 integrin
- in cytotoxicity studies with Cannabis sativa oil extract
- in transfection studies with highly pathogenic avian influenza virus and influenza A virus
Quality
Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
Click here for more information.
Other Notes
Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: > 15
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Effects of cannabis oil extract on immune response gene expression in human small airway epithelial cells (HSAEpC): implications for chronic obstructive pulmonary disease (COPD)
Mamber SW, et al.
Journal of Cannabis Research, 2, 5-5 (2020)
Swantje Thiele et al.
Cell reports, 31(3), 107549-107549 (2020-04-23)
Importin-α adaptor proteins orchestrate dynamic nuclear transport processes involved in cellular homeostasis. Here, we show that importin-α3, one of the main NF-κB transporters, is the most abundantly expressed classical nuclear transport factor in the mammalian respiratory tract. Importin-α3 promoter activity
Articles
Primary human small airway epithelial cells and serum-free medium optimized for the in vitro cultivation of epithelial cells from the distal respiratory system. Protocols for handling, cell passaging, media, and product use.
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