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C0875

Sigma-Aldrich

Calcein

Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Synonym(s):

Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

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About This Item

Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
154071-48-4
Molecular Weight:
622.53
Beilstein:
8181068
UNSPSC Code:
12171500
PubChem Substance ID:
24892279
NACRES:
NA.47

Quality Level

200

description

mixed isomers

form

powder

technique(s)

titration: suitable

color

yellow to brown

solubility

NaOH: 50 mg/mL

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

suitability

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

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Application

Calcein has been used:
  • in whole cell staining
  • in histomorphometry and micro-computed tomography
  • to label active bone-forming surfaces

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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