A8531
Bovine Serum Albumin
pH <5.0, powder
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Recommended Products
biological source
bovine
Quality Level
form
powder
mol wt
~66 kDa
~66,000
packaging
vial of 50 mg
origin
USA origin
technique(s)
ELISA: suitable
gel permeation chromatography (GPC): suitable
immunohistochemistry: suitable
impurities
~0.3% dithiothreitol
pH
<5.0
suitability
suitable for (Suitable for use as a gel filtration marker)
UniProt accession no.
foreign activity
BT virus, none detected
VSV virus, none detected
storage temp.
−20°C
Gene Information
bovine ... ALB(280717)
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General description
Albumin is the most abundant plasma protein in humans. It has a molecular mass of 66.5 kDa.
Application
Albumin from bovine serum has been used:
- for blocking of nonspecific immunoglobulin during immunohistochemical reactions.
- to coat microplates for blocking non-specific binding during I-ELISA (Indirect Enzyme-linked Immunosorbent Assay)
- as molecular mass standard in analytical gel filtration chromatography for Superdex® 75 10/300 GL gel filtration column.
- for measuring fluorescence for PR1 (phytofluor red 1) by using Fluorescence correlation spectroscopy (FCS)
- as a reference mixture along with horse Mb in Size Exclusion Chromatography
Gel filtration molecular weight marker
Biochem/physiol Actions
Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Albumin in serum level is associated with advanced liver disease.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Legal Information
Superdex is a registered trademark of Cytiva
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
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