A3809
Asparaginase from Escherichia coli
lyophilized powder, 100-300 units/mg protein (biuret)
Synonym(s):
L-Asparagine Amidohydrolase
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About This Item
Recommended Products
form
lyophilized powder
Quality Level
specific activity
100-300 units/mg protein (biuret)
composition
Protein, ≥60%
storage temp.
2-8°C
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General description
Asparaginase is a bacterial enzyme and also a chemotherapeutic drug.
Application
Asparaginase from Escherichia coli has been used:
- to compare the cytotoxic effect of L-asparaginase purified from Streptomyces brollosae NEAE-115
- as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues
- to elicit amino acid deprivation
Biochem/physiol Actions
Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle. It is capable of causing pancreatitis in leukemia patients.
Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia . Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.
Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning .
Unit Definition
One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.
Physical form
Lyophilized powder containing sodium chloride
Preparation Note
Chromatographically purified
Analysis Note
Protein determined by biuret.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 2 - Skin Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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