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A0349

Sigma-Aldrich

Anti-l-Afadin antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD03455360
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

canine, mouse, rat, human

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.
microarray: suitable
western blot: 1:2,000 using extract of rat brain

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)

Related Categories

General description

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Specificity

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Application

Anti-l-Afadin antibody produced in rabbit has been used in:
  • western blotting
  • immunofluorescence experiments
  • immunocytochemistry
  • immunohistochemistry

Biochem/physiol Actions

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel nectin-mediated cell adhesion apparatus that is implicated in prolactin receptor signaling for mammary gland development
Kitayama M, et al.
The Journal of Biological Chemistry, 291(11), 5817-5831 (2016)
Genome-wide miRNA analysis identifies miR-188-3p as a novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Pichler M, et al.
Clinical Cancer Research, 23(5), 1323-1333 (2017)
Ching-Hang Wong et al.
Journal of cell science, 117(Pt 5), 783-798 (2004-01-22)
An in vivo model was used to investigate the regulation of tight junction (TJ) dynamics in the testis when adult rats were treated with CdCl(2). It was shown that the CdCl(2)-induced disruption of the blood-testis barrier (BTB) associated with a
Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t (6; 11) chromosome translocation
Prasad R, et al.
Cancer Research, 53(23), 5624-5628 (1993)
Afadin: a key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis
Ikeda W, et al.
The Journal of cell biology, 146(5), 1117-1132 (1999)
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