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S7545

Sigma-Aldrich

Sodium acetate

BioXtra, ≥99.0%

Synonym(s):

Acetic acid sodium salt

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About This Item

Linear Formula:
CH3COONa
CAS Number:
127-09-3
Molecular Weight:
82.03
Beilstein:
3595639
EC Number:
204-823-8
MDL number:
MFCD00012459
UNSPSC Code:
12161700
eCl@ss:
39021906
PubChem Substance ID:
24899754
NACRES:
NA.25

product line

BioXtra

Quality Level

200

Assay

≥99.0%

form

solid

autoignition temp.

1112 °F

impurities

≤0.0005% Phosphorus (P)
<0.01% Insoluble matter

pH

8.5-9.9 (25 °C, 246 g/L)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.002%
sulfate (SO42-): ≤0.003%

cation traces

Al: ≤0.0005%
Ca: ≤0.005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.03%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

SMILES string

[Na+].CC([O-])=O

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

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General description

Sodium acetate is commonly applicable in molecular biology experiments such as nucleic acid purification and precipitation, protein gel electrophoresis, protein crystallization, and high-performance liquid chromatography (HPLC). Sodium acetate is also used in plastic production, dyeing, and leather tanning.

Application

Sodium acetate has been used:
  • in the isolation of DNA from bacterial samples of the human gastrointestinal tract
  • as a component of RNA elution buffer for the preparation of infrared (IR) fluorescent probes in northern blot technique
  • in the lysis buffer for cellular lysis to detect reversible oxidation of protein tyrosine phosphatases (PTPs) with the cysteinyl-labeling assay using IMR90 fibroblasts

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Density, viscosity and ultrasonic velocity studies of aqueous solutions of sodium acetate at different temperatures
Kharat SJ
Journal of Molecular Liquids, 140(1-3), 10-14 (2008)
Christopher Fields et al.
Bio-protocol, 9(8) (2019-08-21)
Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as sensitive as other non-radioactive
Amanda P De Souza et al.
Journal of experimental botany, 66(14), 4351-4365 (2015-04-25)
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Journal of gastroenterology, 49(6), 1026-1039 (2013-07-13)
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