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K4000

Sigma-Aldrich

Kanamycin sulfate from Streptomyces kanamyceticus

Animal Component-free

Synonym(s):

Kanamycin A, Kanamycin sulfate salt

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About This Item

Empirical Formula (Hill Notation):
C18H36N4O11 · H2O4S
CAS Number:
25389-94-0
Molecular Weight:
582.58
EC Number:
246-933-9
MDL number:
MFCD00070253
UNSPSC Code:
51101500
PubChem Substance ID:
24896236
NACRES:
NA.76

biological source

Streptomyces kanamyceticus

Quality Level

200

form

powder

potency

≥750 μg per mg (dry basis)

storage condition

(Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible )

color

white to off-white

solubility

H2O: 50 mg/mL, clear, colorless to faintly yellow

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

Mode of action

protein synthesis | interferes

SMILES string

OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1

InChI key

OOYGSFOGFJDDHP-KMCOLRRFSA-N

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General description

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus.

Application

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used:
  • in the research of bacterial antibiotic resistance
  • in the research on the effect of different antibiotics on bud regeneration

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Features and Benefits

High quality antibiotic suitable for mulitple research applications

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360D

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Extraction and quantitative analysis of aminoglycosides in porcine tissue, using molecular imprinted polymer solid phase extraction followed by LC-MS/MS.

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