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ED2SS

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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About This Item

Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
6381-92-6
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
205-358-3
UNSPSC Code:
12352107
PubChem Substance ID:
24894708
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

200

grade

reagent grade

Agency

suitable for SM 4500 - NH3

Assay

98.5-101.5% (titration)

form

powder

reaction suitability

reagent type: chelator

mp

248 °C (dec.) (lit.)

solubility

H2O: soluble, clear, colorless

application(s)

agriculture
clinical research

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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General description

Ethylenediaminetetraacetic acid (EDTA) disodium salt is an aminopolycarboxylic acid and a hexadentate ligand. It chelates metal cations to form a stable octahedral complex. It is commonly added to buffers, solutions, and even soil samples to control the concentrations of metal ions or to remove metal contaminants. Metal chelators play a crucial role in various research applications, such as biochemistry, molecular biology, and analytical chemistry. For example, EDTA is a blood anticoagulant that chelates calcium ions in the blood, this inhibits clots and is routinely used in hematological assays.

Application

Ethylenediaminetetraacetic acid disodium salt dihydrate has been used as a component of the plasma membrane resuspension buffer, inhibitor′s preservative solution (IS) and luciferase activity assay mix.
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

Reagent grade for general lab use

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H332,H373

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Disodium edetate European Pharmacopoeia (EP) Reference Standard

D2900000

Disodium edetate

Ethylenediaminetetraacetic acid disodium salt dihydrate Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1068

Ethylenediaminetetraacetic acid disodium salt dihydrate

Edetate disodium United States Pharmacopeia (USP) Reference Standard

USP

1233009

Edetate disodium

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid ≥97.0%

Sigma-Aldrich

E4378

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

Titriplex® III for analysis, ACS reagent, ISO, reag. Ph. Eur.

Supelco

1.08418

Titriplex® III

Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes
Antonelli G, et al.
Clinical Biochemistry, 40(8), 545-550 (2007)
Isolation of plasma membrane-associated membranes from rat liver
Suski JM, et al.
Nature Protocols, 9(2), 312-312 (2014)
Conazole fungicides inhibit Leydig cell testosterone secretion and androgen receptor activation in vitro.
Roelofs MJE, et al.
Toxicology Reports, 1, 271-283 (2014)
Ethylenediaminetetraacetic acid induces antioxidant and anti-inflammatory activities in experimental liver fibrosis
Gonzalez-Cuevas J, et al.
Redox Report, 16(2), 62-70 (2011)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)
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Protocols

Enzymatic Assay and ATP Sensitivity Test of Luciferase

Objective: To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Superoxide Dismutase

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