Skip to Content
Merck
All Photos(5)

Documents

D9805

Sigma-Aldrich

Fast Blue B Salt

Dye content ~95 %

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

Sign Into View Organizational & Contract Pricing
All Photos(5)

About This Item

Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
14263-94-6
Molecular Weight:
475.47
Colour Index Number:
37235
Beilstein:
1499067
EC Number:
238-153-2
MDL number:
MFCD00075203
UNSPSC Code:
12171500
PubChem Substance ID:
24894113
NACRES:
NA.47

description

Technical grade

Quality Level

200

form

powder

composition

Dye content, ~95%

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

Pictograms

FlameHealth hazard
GHS02,GHS08

Signal Word

Danger

Hazard Statements

H242 - H350

Hazard Classifications

Carc. 1B - Self-react. C

Storage Class Code

5.2 - Organic peroxides and self-reacting hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 4

1 of 4

1-Naphthyl acetate ≥98% (C)

Sigma-Aldrich

N8505

1-Naphthyl acetate

Fast Black K Salt hemi(zinc chloride) salt practical grade

Sigma-Aldrich

F7253

Fast Black K Salt hemi(zinc chloride) salt

Cobalt(II) thiocyanate 96%

Sigma-Aldrich

216135

Cobalt(II) thiocyanate

Fast Corinth V zinc chloride double salt Dye content ≥90 %

Sigma-Aldrich

227366

Fast Corinth V zinc chloride double salt

Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata).
Nolasco H, et al.
Fish Physiology and Biochemistry, 37, 43-43 (2011)
Dong Ho Kang et al.
Polymers, 12(11) (2020-11-18)
In this study, composite anion exchange membranes (AEMs) were synthesized by cross-linking poly(phenylene oxide) (PPO) with cellulose functionalized by 1,4-diazabicyclo[2.2.2]-octane (DABCO) or di-guanidine (DG). The structural and morphological characteristics of the synthesized AEMs were characterized by FTIR, 1H-NMR, SEM, TEM
A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
Sim?es-Pires CA, et al.
Phytochemical Analysis, 20, 511-511 (2009)
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
Sartory DP, et al.
Letters in Applied Microbiology, 42, 418-418 (2006)
A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.
Marston A, et al.
Phytochemical Analysis, 13, 51-51 (2002)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service