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Sigma-Aldrich

Basic Fuchsin

certified by the Biological Stain Commission, Dye content ≥88 %

Synonym(s):

Basic Parafuchsin, Basic Red 9, Magenta™ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C19H17N3 · HCl
CAS Number:
569-61-9
Molecular Weight:
323.82
Colour Index Number:
42500
Beilstein:
4164603
EC Number:
209-321-2
MDL number:
MFCD00001657
UNSPSC Code:
12171500
PubChem Substance ID:
24888442

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥88%

color

green to dark green

pH

1.0-3.1, purple to red

mp

268-270 °C (dec.) (lit.)

λmax

544 nm

ε (extinction coefficient)

≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L
≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

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Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Suitability

Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

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Plant Fibers (2012)
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The intimate association of host and fungus in arbuscular mycorrhizal (AM) symbiosis can potentially trigger induction of host defence mechanisms against the fungus, implying that successful symbiosis requires suppression of defence. We addressed this phenomenon by using AM-defective vapyrin (vpy)
Bisen PS
Laboratory Protocols in Applied Life Sciences (2014)
Sharma A
Plant Chromosomes (1999)
I A Schrijver et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(1), 95-103 (2000-02-01)
Integral immunohistochemical analysis of immune responses in frozen sections requires that, in addition to constitutively expressed membrane CD markers, less stable determinants can be reliably visualized. Therefore, we compared the commonly used acetone fixation method with pararosaniline fixation for six
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