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114421

Sigma-Aldrich

Bromothymol Blue sodium salt

ACS reagent

Synonym(s):

3′,3′′-dibromothymolsulfonephthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
34722-90-2
Molecular Weight:
646.36
Beilstein:
6887926
EC Number:
252-169-7
MDL number:
MFCD00077263
UNSPSC Code:
12171500
PubChem Substance ID:
24847172
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

form

crystalline powder
powder or crystals

technique(s)

titration: suitable

visual transition interval

6.0-7.6, yellow to blue

solubility

water: 0.1%, clear to very slightly hazy

λmax

392 nm
615 nm

ε (extinction coefficient)

≥35000 at 614-618 nm in 0.1 M NaOH at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

SMILES string

CC1=C(C=C(C(=C1Br)O)C(C)C)C(=C2C=C(C(=O)C(=C2C)Br)C(C)C)C3=CC=CC=C3S(=O)(=O)[O-].[Na+]
[Na+].CC(C)c1cc(c(C)c(Br)c1O)C(=C2\C=C(C(C)C)C(=O)C(Br)=C2C)\c3ccccc3S([O-])(=O)=O

InChI

InChI=1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M
NMKFVGALBGZKGW-FKWCIMQXSA-M

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Application

Bromothymol Blue has been used for freshwater pH measurements over a broad range of buffer intensities.

Biochem/physiol Actions

Bromothymol Blue is a pH-indicator. It is also used for carbonic anhydrase assay due to the conversion of carbon dioxide to bicarbonate.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Hydrophilic region of lecithin membranes studied by bromothymol blue and effects of an inhalation anesthetic, enflurane.
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Proceedings of the National Academy of Sciences of the USA, 76, 5114-5118 (1979)
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