CSPD-RO
Roche
CSPD ready-to-use
Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate
Synonym(s):
ready-to-use, cspd
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About This Item
UNSPSC Code:
41105500
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Quality Level
Assay
>98% (HPLC)
form
solution
mol wt
Mr 461
packaging
pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)
manufacturer/tradename
Roche
storage temp.
2-8°C
Related Categories
General description
CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
Application
CSPD ready-to-use has been used for:
- the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
- colony and plaque hybridization
- gel shift assay
- sequencing
Features and Benefits
- Save time with a fast, highly sensitive substrate.
- Easily strip and reprobe membranes.
- Take multiple exposures of up to two days for each experiment.
- Reuse the substrate by filtering and storing in sodium azide.
Physical form
25mM solution (11.6mg/ml), 100x concentrated, colorless solution.
Preparation Note
Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Other Notes
For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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