11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Synonym(s):
RNA labeling
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About This Item
UNSPSC Code:
41105500
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form
solution
Quality Level
usage
sufficient for 20 reactions
packaging
pkg of 40 μL
manufacturer/tradename
Roche
technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
storage temp.
−20°C
Related Categories
General description
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Specificity
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Application
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Quality
The mix is function tested in combination with the DIG RNA Labeling Kit.
Principle
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Preparation Note
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
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