11209264001
Roche
DNA Molecular Weight Marker VII
pkg of 50 μg (in 200 μl), solution
Synonym(s):
DNA marker
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About This Item
UNSPSC Code:
41105335
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form
solution
Quality Level
packaging
pkg of 50 μg (in 200 μl)
manufacturer/tradename
Roche
concentration
250 μg/mL
color
colorless
solubility
water: miscible
storage temp.
−20°C
Related Categories
General description
Contents
Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).
Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).
Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I.
The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.
Size Range: 0.081 to 8.57 kbp
The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.
Size Range: 0.081 to 8.57 kbp
Application
DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
Sequence
The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.
Unit Definition
50 μg = 1A260 unit
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed store at 2 to 8 °C.
Once thawed store at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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