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10567752001

Roche

Alkaline Phosphatase

EIA grade, from calf intestine

Synonym(s):

AP, Alkaline Phosphatase

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About This Item

Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

bovine (calf) intestine

Quality Level

100

form

solution

specific activity

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

packaging

pkg of 1.5 mL (15 mg)

manufacturer/tradename

Roche

optimum pH

9.8

pH range

9.0-11.0

shipped in

wet ice

Related Categories

General description

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

Application

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

Quality

Purity: single peak (HPLC)

Sequence

Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.

Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.

NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.

Unit Definition

Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]

Physical form

Solution, ready-to-use, 10mg/ml

Preparation Note

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.

Storage and Stability

Store at 2 to 8 °C. (Do not freeze)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yupeng Li et al.
Chemical research in toxicology, 34(4), 992-1003 (2021-03-12)
The tobacco-specific nitrosamines N'-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are considered to be two of the most important carcinogens in unburned tobacco and its smoke. They readily cause tumors in laboratory animals and are classified as "carcinogenic to humans" by the

Protocols

Alkaline Phosphatase Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

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