SCM013
NDiff Neuro-27 Medium Supplement (100x)
NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
Recommended Products
Quality Level
form
liquid
manufacturer/tradename
Chemicon®
Millipore
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
shipped in
dry ice
General description
NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants and other factors that has been specifically developed for the in vitro propagation and maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium (Nichols J, 2006). This product can also be used to differentiate murine ES cells into post-mitotic neurons particularly via monolayer differentiation (Ying QL, 2003).
Physical form
NDiff Neuro-27 Medium Supplement is supplied as an aqueous solution. Optimal working concentration of the medium supplement must be determined for specific uses and cell types. In most instances, a 1:40-1:200 dilution is appropriate. Typically, Neuro-27 is diluted 1:100 for self renewal and neural differentiation of mouse ES cells.
Storage and Stability
Maintain at -20 °C until expiration date. Protect from light. Once thawed, aliquot unused portion and store at -20 °C until future use. Once added to the cell culture medium, the product is stable at 4 °C for four weeks.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Customers Also Viewed
Qi-Long Ying et al.
Nature biotechnology, 21(2), 183-186 (2003-01-14)
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceutical screening or transplantation is
Derivation and propagation of embryonic stem cells in serum- and feeder-free culture
Nichols, Jennifer and Ying, Qi-Long
Methods in Molecular Biology, 329, 91-98 (2006)
Defined conditions for neural commitment and differentiation
Ying, Qi-Long and Smith, Austin G
Test, 365, 327-341 (2003)
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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