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MABT167

Sigma-Aldrich

Anti-Transgelin/SM22a Antibody, clone 20F2.2

clone 20F2.2, from mouse

Synonym(s):

Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, SM22a

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

20F2.2, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001001522

UniProt accession no.

Q01995

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TAGLN(6876)

General description

Transgelin, also known as 22 kDa actin-binding protein, Protein WS3-10, and Smooth muscle protein 22-alpha (SM22-alpha), is an actin cross-linking protein that is involved in cellular calcium interactions and contractile properties of the cytoskeleton contributing to replication and senescence in smooth muscle tissue. Transgelin is overexpressed in senescent human fibroblasts.

Immunogen

GST-tagged recombinant protein corresponding to human Transgelin/SM22a.

Application

Anti-Transgelin/SM22a Antibody, clone 20F2.2 is a highly specific mouse monoclonal antibody, that targets Transgelin & has been tested in western blotting & ICC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western Blotting Analysis: 1 µg/ml from a representative lot detected Transgelin/SM22a in 10 µg of NIH/3T3 and PC12 cell lysates.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Transgelin/SM22a in NIH/3T3 cells.

Quality

Evaluated by Western Blotting in U20S cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Transgelin/SM22a in 10 µg of U20S cell lysate.

Target description

~22 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
U20S cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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