MAB13431
Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2
clone A-Gel VC2, Chemicon®, from mouse
Synonym(s):
Gelatinase A, 72 kDa Type IV Collagenase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
A-Gel VC2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MMP2(4313)
General description
MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.
Specificity
MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.
CELLULAR LOCALIZATION:
Cytoplasmic.
CELLULAR LOCALIZATION:
Cytoplasmic.
Immunogen
Epitope: pro and active form
Human APMA-activated native 72kDa Gelatinase A.
Application
Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).
Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.
ELISA (not suitable for coating, contains BSA) 1:200-1:2000
Not suitable for Western blotting
Optimal working dilution should be determined by the end user.
ELISA (not suitable for coating, contains BSA) 1:200-1:2000
Not suitable for Western blotting
Optimal working dilution should be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Linkage
Replaces: 04-1048
Physical form
10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide
Format: Purified
Storage and Stability
Antibody is stable for 12 months when stored at 2-8ºC
Analysis Note
Control
POSTIVE CONTROL:
Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.
POSTIVE CONTROL:
Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Marano, G; Gronewold, C; Frank, M; Merling, A; Kliem, C; Sauer, S; Wiessler, M; Frei et al.
Beilstein Journal of Organic Chemistry null
Expression and activity of matrix metalloproteinases-2 and -9 in serum, core needle biopsies and tissue specimens of prostate cancer patients.
Christian G Sauer, Alexandra Kappeler, Monika Spath, Jens J Kaden, Maurice S Michel et al.
Virchows Archiv null
Decreased RECK and Increased EMMPRIN expression in urothelial carcinoma of the bladder are associated with tumor aggressiveness.
Wittschieber, D; Stenzinger, A; Klauschen, F; Stephan, C; Jung, K; Erbersdobler, A; Rabien, A
Pathobiology : journal of immunopathology, molecular and cellular biology null
A B Frey et al.
Histopathology, 50(6), 720-726 (2007-05-12)
Osteopontin (OPN) is a matricellular protein involved in tissue remodelling, cell-mediated immunity and malignant transformation. High OPN serum levels predict poor prognosis in non-small cell carcinoma and set patients with malignant pleural mesothelioma (MM) apart from disease-free asbestos-exposed individuals. Yet
Bartlomiej Perek et al.
BioMed research international, 2013, 730721-730721 (2013-10-24)
Migration of the smooth muscle cells (SMCs) to the tunica media in the saphenous vein (SV) transplants is facilitated by matrix metalloproteinases (MMPs). The aim of this study was to identify any associations between expression of MMP-2 or endogenous tissue
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