IEVH85R
Immobilon® -E PVDF Membrane
1 roll, 8.5 cm × 10 m, 0.45 µm pore size, transfer membrane wets out in transfer buffer or water
Synonym(s):
Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane
About This Item
Recommended Products
product name
Immobilon®-E PVDF Membrane, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
material
PVDF membrane
plain filter
white filter
Quality Level
feature
easily wetted with water
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
filter L × W
8.5 cm × 10 m
pore size
0.45 μm pore size
compatibility
for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination
detection method
chemiluminescent
colorimetric
radioactive
shipped in
ambient
Related Categories
General description
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.
Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Legal Information
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Protocols
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service