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AC111B

Sigma-Aldrich

Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate

from sheep, biotin conjugate

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

sheep

Quality Level

conjugate

biotin conjugate

antibody form

purified antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

technique(s)

immunohistochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Specificity

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.

Immunogen

Purified mouse IgG.

Application

Immunohistochemistry Analysis: A representative lot was validated by immunohistochemistry staining (1:500 dilution) of human liver tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate is an antibody against Sheep Mouse IgG for use in Immunohistochemistry.

Quality

Evaluated by Immunohistochemistry in human prostate tissue.

Immunohistochemistry Analysis: Performance of this biotin-conjugated secondary antibody has been validated by immunohistochemistry staining (1:500 dilution) of human prostate tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).

Target description

~150 kDa observed.

Physical form

Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to biotin conjugation.
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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