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ABN207

Sigma-Aldrich

Anti-DBN1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Drebrin, Developmentally-regulated brain protein, DBN1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, horse, human, rat

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_004386

UniProt accession no.

Q16643

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DBN1(1627)

General description

Drebrin (Developmentally-regulated brain protein) is also known by the gene name DBN1. Drebrins are found in the cytoplasm of brain neurons as well as tissues including heart, placenta skeletal muscle kidney and pancreas. Debrin binds F-actin and is involved in the mechanisms of development, cell migration, neuronal processes and dendrite plasticity.

Immunogen

KLH-conjugated linear peptide corresponding to human DBN1.

Application

Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected DBN1 in human cerebellum and rat midbrain tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This DBN1 antibody is validated for use in WB & IHC for the detection of the DBN1 protein.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected DBN1 in 10 µg of mouse brain tissue lysate.

Target description

~110 kDa observed. The calculated molecular weight is 71 kDa, however DBN1 has been shown as a ~110 kDa band in western blotting (Maezawa, I., et al. (2006). Novus Biologicals. 1-2).

Linkage

Replaces: AB15288

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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