Anti-Orexin-1 Receptor Antibody

Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Recognizes Rat and Human Orexin-1.

A 16 aa peptide near the N-terminus of rat OX1R and a 16 aa peptide near the C-terminus of human OX1R.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

This Anti-Orexin-1 Receptor Antibody is validated for use in ELISA, WB for the detection of Orexin-1 Receptor.

Western blot: 1-10 μg/mL using Chemiluminescence technique. Protein runs at 45-48 kDa.

ELISA: 1:10,000 -1:100,000 against 50-100 ng/wel immunogen peptide.

Optimal working dilutions must be determined by end user.

45-48 kDa

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

ImmunoAffinity Purified

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Hypothalamus

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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