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biological source
bovine
Quality Level
recombinant
expressed in E. coli
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
technique(s)
targeted protein degradation: suitable
suitability
suitable for enzyme test
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
General description
Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.
Components
•50 URecombinant Enterokinase
•2 ml1X rEK Dilution/Storage Buffer
•1 ml10X rEK Cleavage Buffer
•10 µgCleavage Control Protein
•2 ml1X rEK Dilution/Storage Buffer
•1 ml10X rEK Cleavage Buffer
•10 µgCleavage Control Protein
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Unit Definition
One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.
Other Notes
Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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