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345806-M

Sigma-Aldrich

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

Synonym(s):

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli, β1,4-D-Galactoside Galactohydrolase

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About This Item

CAS Number:
9031-11-2
Enzyme Commission number:
3.2.1.23 (BRENDA, IUBMB)
UNSPSC Code:
12352202

recombinant

expressed in E. coli

Quality Level

200

form

liquid

specific activity

≥1.5 units/mL
≥6 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected

storage temp.

2-8°C

General description

Note: 1 mU = 1 milliunit.
Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.

Other Notes

Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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