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Sigma-Aldrich

GC7

>95% (NMR), powder, deoxyhypusine synthase inhibitor, Calbiochem®

Synonym(s):

Deoxyhypusine Synthase Inhibitor, GC7, N1-guanyl-1,7-diamine-heptane, 1-(7-ammonioheptyl)guanidinium sulfate

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About This Item

Empirical Formula (Hill Notation):
C8H22N4O4S
CAS Number:
150333-69-0
Molecular Weight:
270.35
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Deoxyhypusine Synthase Inhibitor, GC7, The Deoxyhypusine Synthase Inhibitor, GC7, also referenced under CAS 150333-69-0, controls the biological activity of Deoxyhypusine Synthase.

Quality Level

100

Assay

>95% (NMR)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

color

white

solubility

water: 25 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

InChI

1S/C8H20N4/c9-6-4-2-1-3-5-7-12-8(10)11/h1-7,9H2,(H4,10,11,12)

InChI key

YAOAMZOGXBMLFQ-UHFFFAOYSA-N

General description

A cell-permeable inhibitor that targets the spermidine-binding site of Deoxyhypusine Synthase (Ki = 9.7 nM), an enzyme which is responsible for the first step hypunisation of eukaryotic initiation factor 5A (eIF5A). In vivo, it demonstrates almost complete cessation of hypusine formation in CHO cells at ≥ 1 µM. Several studies indicate this compound as a useful tool for the down-regulation of eIF5A, a protein essential for cell proliferation. For example, it exhibits an anti-proliferative effect on both BCR-ABL -positive and -negative leukemia cell lines (K562 and HL-60) dose-dependently, at concentrations between 5 µM and 40 µM. In addition, it is shown to significantly impair the growth and invasion of primary HCC cells (HepG2 and Hep3B) in the 10-20 µM range, and against non-tumorigenic CL-48 cells at concentrations as high as 40 µM, with no apparent cytotoxicity. At 25 µM, GC7 treatment is shown to reverse eIF5a-mediated Skeletal Muscle Stem Cell Differentiation.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lee, N.P., et al. 2010. Int J Cancer.4, 968.
Luchessi, A., et al. 2008. J Cell Physiol.3, 480.
Balabanov, S., et al. 2007. Blood.4, 1701.
Jakus, J., et al. 1993. J Biol Chem.18, 13151.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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