251500-M
DAF-2
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Synonym(s):
DAF-2, 4,5-Diaminofluorescein
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About This Item
Empirical Formula (Hill Notation):
C20H14N2O5
CAS Number:
Molecular Weight:
362.34
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.25
Recommended Products
Quality Level
Assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
pale yellow
fluorescence
λex ~495 nm
λem ~515 nm
storage temp.
−20°C
InChI
1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
InChI key
LTYUPYUWXRTNFQ-UHFFFAOYSA-N
General description
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
Biochem/physiol Actions
Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Warning
Toxicity: Irritant (B)
Physical form
A 5 mM (1 mg in 550 µl) solution in DMSO.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Legal Information
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
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