Fractogel^® EMD SO₃⁻ (S) Resin

5 x 30 mm

RoboColumn Fractogel^® SO3 (S), 0.6ml enables:

• Efficient separation between monomer, dimer, and high molecular weight aggregates
• High binding capacity
• High resolution
• Efficient polishing step
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Plastic bag(s)

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany