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1.16464

Supelco

HPTLC plates, Silica gel 60 CN F₂₅₄s

pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support

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About This Item

UNSPSC Code:
41115711

material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

10 cm × 10 cm

particle size

5-6 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

25 Glass plates 10 x 10 cm

Linkage

Replaces: 16464-5; 16464

Analysis Note

Specific surface area (BET): 460 - 520 m²/g
Pore volume: 0.71 - 0.78 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Steroidtest, straight phase
hRf-values
- hydrocortisone, straight phase:
10 - 20
- Reichstein S, straight phase: 20 - 30
- Methyltestosterone, straight phase: 35 - 47
B) Steroid test, reversed phase
- methyltestosterone, steroid test: 36 - 48
- Reichstein S, steroid test: 46 - 58
- hydrocortisone, steroid test: 56 - 68
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) petroleum benzine 40-60°/acetone (80/20 v/v)
Eluent b) acetone/water (30/70 v/v)

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