Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.

This antibody recognizes RNA polymerase II subunit B1 CTD when phosphorylated at Ser2.

Epitope: Ser2

Ovalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser2.

Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 (phospho CTD Ser-2) has been validated in WB, ELISA.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins

Evaluated by Western Blot in γ-PPase untreated and treated NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.

~ 220 kDa

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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