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HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (15 °C)

HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (15 °C) application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

12024AST

Astec® CHIROBIOTIC® T Chirale HPLC-Säule

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

H6034

L-Histidin

suitable for cell culture, meets EP, USP testing specifications, from non-animal source

H8000

L-Histidin

ReagentPlus®, ≥99% (TLC)

H3911

L-Histidin

73767

L-Histidin

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Vorsäulenkartusche

Produkt-Nr.
Beschreibung
Preisangaben

12100AST

Astec® Chirobiotic® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.006, A:B:C)

flow rate

0.5 mL/min

column temp.

15 °C

detector

UV, 205 nm

injection

10 μL

sample

200 μg /mL in 50:50 water:methanol

Beschreibung

Rechtliche Hinweise

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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