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Merck

T1706

Sigma-Aldrich

TransIT-CRISPR®

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About This Item

UNSPSC-Code:
12352200

Anwendung(en)

CRISPR

Versandbedingung

wet ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Leistungsmerkmale und Vorteile

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Komponenten

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent

Verlinkung

To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Sonstige Hinweise

  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Rechtliche Hinweise

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Piktogramme

FlameExclamation mark

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Eye Irrit. 2 - Flam. Liq. 2

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 1

Flammpunkt (°F)

57.2 °F - closed cup

Flammpunkt (°C)

14.0 °C - closed cup


Analysenzertifikate (COA)

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Protokolle

Sigma-Aldrich® sgRNA, crRNA and tracrRNA come HPLC purified as standard with our performance guarantee—even for custom designs.

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Verwandter Inhalt

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Sigma-Aldrich® Advanced Genomics ist der führende Anbieter von Technologien für die Geneditierung und Genstummschaltung wie CRISPR, Cas9, Single Guide-RNA (sgRNA) und Zinkfingernuklease (ZFN).

Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes with high on-target cleavage efficiency fused to Enhanced Green Fluorescent Protein (EGFP) for real-time observation of Cas9 delivery and clearance from cells.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.