Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

SRP3292

Sigma-Aldrich

R-SPONDIN-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(e):

Rspo1

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

Biologische Quelle

human

Rekombinant

expressed in CHO cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

Form

lyophilized

Wirksamkeit

1.0-3.0 μg/mL ED50

Mol-Gew.

26.0-40.0 kDa

Verpackung

pkg of 20 μg

Verunreinigungen

<0.1 EU/μg endotoxin, tested

Farbe

white

UniProt-Hinterlegungsnummer

Q2MKA7

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... RSPO1(284654)

Verwandte Kategorien

Allgemeine Beschreibung

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the (Rspo) family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Recombinant human R-Spondin-1 is a 26.7kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0kDa by SDS PAGE analysis under reducing conditions.

Biochem./physiol. Wirkung

Rspo (R-Spondin) can interact with the Frizzled/LRP6 (LDL receptor related protein 6) receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Disruption of Rspo-1 gene causes recessive syndrome with XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Rspo-1 is also associated with radioresistance of tumor cells.

Physikalische Form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kunden haben sich ebenfalls angesehen

Slide 1 of 7

1 of 7

A&nbsp;83-01 &#8805;98% (HPLC)

Sigma-Aldrich

SML0788

A 83-01

N-Acetyl-L-Cystein suitable for cell culture, BioReagent

Sigma-Aldrich

A9165

N-Acetyl-L-Cystein

[Leu15]-Gastrin I Human &#8805;95% (HPLC)

Sigma-Aldrich

G9145

[Leu15]-Gastrin I Human

Nicotinamid BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

N0636

Nicotinamid

Sigma-Aldrich

Sigma-Aldrich

SRP3027

EGF human

Sigma-Aldrich

Sigma-Aldrich

A8301

Albumin, glycated human

Transforming Growth Factor-&#946;1 human TGF-&#946;1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

H8541

Transforming Growth Factor-β1 human

R-Spondin proteins: a novel link to beta-catenin activation.
Kim KA, et al.
Cell Cycle, 5, 23-26 (2006)
R-spondin1 is essential in sex determination, skin differentiation and malignancy.
Parma P, et al.
Nature Genetics, 38, 1304-1309 (2006)
Xuefeng Gu et al.
Oncotarget, 6(12), 9756-9765 (2015-04-14)
Although radiation therapy is the most effective postoperative adjuvant treatment, it does not substantially improve the long-term outcomes of glioma patients because of the characteristic radioresistance of glioma. We found that R-Spondin1 (Rspo1) expression was elevated in high-grade gliomas and
Minke E Binnerts et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(37), 14700-14705 (2007-09-07)
The R-Spondin (RSpo) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. We have previously shown that RSpo proteins can induce proliferative effects on the gastrointestinal epithelium in mice. Here we provide a mechanism whereby RSpo1
Yong-Guo Zhang et al.
Autophagy, 15(11), 1935-1953 (2019-03-22)
Extracellular HMGB1 (high mobility group box 1) is considered as a damage-associated molecular pattern protein. However, little is known about its intracellular role. We studied the mechanism whereby intestinal epithelial HMGB1 contributes to host defense, using cell culture, colonoids, conditional

Artikel

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.