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SRP0186

Sigma-Aldrich

Bcl-2 Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(e):

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell leukemia/lymphoma 2

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Biologische Quelle

human

Rekombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

Form

aqueous solution

Mol-Gew.

27 kDa

Verpackung

pkg of 100 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Konzentration

>0.02 mg/mL

NCBI-Hinterlegungsnummer

P10415

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... BCL2(596)

Allgemeine Beschreibung

B-cell lymphoma 2 (BCL-2) is an anti-apoptotic and target gene of nuclear factor-κB (NF-κB). The protein is expressed in the nuclear envelope, mitochondria and endoplasmic reticulum. The gene encoding BCL-2 is localized on human chromosome 18q21. Human Bcl-2 (amino-acids 1-211) (GenBank Accession No. P10415) with C-terminal His tag, MW = 27 kDa, expressed in an E. coli expression system.

Anwendung

Useful for the study of apoptosis, screening inhibitors and selectivity profiling.

Biochem./physiol. Wirkung

B-cell lymphoma 2 (BCL-2) inhibits apoptosis in cells and has been shown to be upregulated in adenomas.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Onursal Onen et al.
Sensors (Basel, Switzerland), 12(9), 12317-12328 (2012-11-01)
Changes in mass loading on the surface of acoustic biosensors result in output frequency shifts which provide precise measurements of analytes. Therefore, to detect a particular biomarker, the sensor delay path must be judiciously designed to maximize sensitivity and specificity.
Maria Lauda Tomasi et al.
Oncotarget, 6(35), 37706-37723 (2015-09-30)
Ubiquitin-conjugating enzyme 9 (Ubc9) is required for sumoylation and inhibits apoptosis via Bcl-2 by unknown mechanism. Methionine adenosyltransferase 2A (MAT2A) encodes for MATα2, the catalytic subunit of the MATII isoenzyme that synthesizes S-adenosylmethionine (SAMe). Ubc9, Bcl-2 and MAT2A expression are
Rr Suzy Indharty
Asian journal of neurosurgery, 8(2), 83-89 (2013-09-21)
Traumatic brain injury (TBI) is one of the major causes of death and disability. Apoptosis after TBI contributes significantly to the final extent of tissue damage. The Bcl-2 family proteins are important apoptosis modulators which increased in injured neurons. Bcl-2
Christelle Villeneuve et al.
Molecular biology of the cell, 22(7), 999-1013 (2011-02-04)
Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action remains incompletely understood. Deciphering the network of Bcl-2 interacting factors is necessary to better understand the key function of Bcl-2 in apoptosis initiation. To identify novel
P. Riederer
Advances in Research on Neurodegeneration, 5 null
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